7 Their capacity to target specific signaling pathways might reduce side effects to normal tissue and be an approach to generate specific antitumor agents. 5, 6 Apoptosis induced by Shiga-like toxins can be associated with enhanced expression of pro-apoptotic protein and inhibits expression of antiapoptosis. 4, 5 The high specificity and apoptosis-inducing properties of Shiga or Shiga-like toxins suggest these may potentially be used for the treatment of Gb3-expressing cancer. 2, 3 Researchers always search for new effective agents with as small side effects as possible to treat cancer.įurthermore, Shiga or Shiga-like toxins as immunotherapeutic agents may be used against tumor malignancies, either independently or in combination with chemotherapy or radiotherapy. Besides surgery, endocrine treatment, radiation, and chemotherapy treatment, immunotherapy has become an established part of systemic therapy. 1 However, these often have substantial side effects. Several single-agent and combination chemotherapeutic treatments have been shown to be effective in the management of the metastatic disease. coli O157:H7 local isolate (Indonesia) has potential as a proapoptotic and/or necrotic agent for treating T47 breast cancer cell lines, as effectively as ATCC 43894 control isolates.īreast cancer is the most common malignancy affecting women in the Western world. These titers were lower than the control isolate ATCC 43894, but they had a necrosis effect higher (P P > .05) with the control isolate ATCC 43894, ie, 13.0% from 13.3%.ConclusionThis study concludes that the Shiga toxin produced by E. Toxin production of each isolate was detected using a sandwich enzyme-linked immunosorbent assay, and the treatment of cell lines was observed for 24 hours, with 2 replications 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide tests and acridine orange/ethidium bromide double staining assays were used for detection and analyses of apoptosis.ResultsThe study showed 2 local strains of E.
coli O157:H7 as a proapoptosis agent on the T47 breast cancer cell line.MethodsAs many as 30 culture cells of T47D breast cancer cell line were subjected to purified extracts of Shiga-like toxin originating from 5 local isolates of E. The study aimed to analyze local strains of E. In addition, the antitumor potency of local isolates from Indonesia, which are known to be less toxic than the control isolate ATCC 43894, has not yet been tested. PurposeIt has been suggested that Shiga-like toxins produced by Escherichia coli O157:H7 could be used as novel therapeutic agents against malignant tumors.